ReproSil SEC - for Size Exclusion | |
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SEC |
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Packing: | Pore size: | MW of Proteins: | MW of Linear Molecules: | ||
(denaturated proteins, peptides, other org. Polymers) | |||||
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Reprosil 50 SEC | 50 A° | 8 000 - 30 000 D | 500 - 10 000 D | ||
Reprosil 200 SEC | 200 A° | 10 000 - 500 000 D | 2 000 - 70 000 D | ||
Reprosil SEC 4000 | 450 A° | 20 000 - 5 000 000 D | 4 000 - 500 000 D | ||
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Column: ReproSil-Pur 50 SEC, 5µm (300 x 8 mm) Flow: 0,5 ml/min. Eluent: 50 mM KH2PO4+100mM KCL, pH6,5 Peptide Sample: 1= MW: 3894 D 2= MW: 1593 D 3= MW: 826D |
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Column: Reprosil 200 SEC, 5 µm (300 x 8 mm) Flow: 1 ml / min Eluent: 50 mM KH2PO4+ 150 mM KCL, pH 7.0 Sample: 1 =Thyroglobulin (MW - 670 KD) 2 = Ovalbumin (MW - 44 KD) 3 = Myoglobulin (MW - 17 KD 4 = Phenylalanine |
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Column: Reprosil SEC 4000, 5 µm A.) 250 x 4.6 mm, Flow: 1 ml / min B.) 750 x 4.6 mm, Flow: 0,33 ml / min Eluent: 50 mM KH2PO4+ 150 mM KCL, pH 7.0 Sample: 1= Thyroglobulin (MW - 670 KD) 2= Ovalbumin (MW - 44 KD) 3= Phenylalanine |
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ReproSil 200 SEC Typical features for ReproSil 200 SEC, 5 µm (Mat..No.: R25.sec.) pH-Stability: 2 - 7.5 Pore size: 200 A° Pore volume: 1.2 ml/g Capacity: about 10 - 500 µg, depending on column-dimension and protein Max. pressure: about 200 bars |
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Dimension: 300 x 8 mm, Eluent: (for all shown separations) 50 mM K-phosphate-buffer, pH=7 + 150 mM KCL, Flow: 1 ml/min., Max.Pressure: 200 bars | |||||
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GPC |
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Polymeric phases for organic GPC: | |||||
Typical dimensions: 300 x 8 mm or 600 x 8 mm iD. Other dimensions on request. | |||||
for non aquous GPC | Daltons | ||||
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ReproGel PS GPC, 5 µm, 50 A° | < 1 000 | rg0.050. | |||
ReproGel PS GPC, 5 µm, 100 A° | > 3000 | rg0.100. | |||
ReproGel PS GPC, 5 µm, 500 A° | > 20 000 | rg0.500. | |||
ReproGel PS GPC, 5 µm, 1000 A° | 1 000 - 40 000 | rg0.103. | |||
ReproGel PS GPC, 5 µm,10 000 A° | 4 000 - 500 000 | rg0.104. | |||
ReproGel PS GPC, 5 µm, 100 000 A° | 10 000 - 2 000 000 | rg0.105. | |||
ReproGel PS GPC, 5 µm, 1 000 000 A° | 200 000 - 10 000 000 | rg0.106. | |||
ReproGel PS GPC, 5 µm, linear | 1 000 - 1 000 000 | rg0. lin. | |||
Available particle sizes: 3, 5, 10 µm | |||||
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