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ReproSil SEC - for Size Exclusion

Reprosil SEC phases are designed for the Gel Filtration Chromatography (GFC) of proteins, peptides and
other org. polymers in aqueous systems. In GFC elution order is a function of molecular weight, the largest
molecules eluting first and the smallest last.
The Silica-based Reprosil SEC packings are available with 3 different pore sizes:

References of ReproSil SEC


*SEC*
Packing:	Pore size:	MW of Proteins:	MW of Linear Molecules:
			(denaturated proteins, peptides, other org. Polymers)

Reprosil 50 SEC	50 A°	8 000 - 30 000 D	500 - 10 000 D
Reprosil 200 SEC	200 A°	10 000 - 500 000 D	2 000 - 70 000 D
Reprosil SEC 4000	450 A°	20 000 - 5 000 000 D	4 000 - 500 000 D


			Column: ReproSil-Pur 50 SEC, 5µm (300 x 8 mm) Flow: 0,5 ml/min. Eluent: 50 mM KH2PO4+100mM KCL, pH6,5 Peptide Sample: 1= MW: 3894 D 2= MW: 1593 D 3= MW: 826D

			Column: Reprosil 200 SEC, 5 µm (300 x 8 mm) Flow: 1 ml / min Eluent: 50 mM KH2PO4+ 150 mM KCL, pH 7.0 Sample: 1 =Thyroglobulin (MW - 670 KD) 2 = Ovalbumin (MW - 44 KD) 3 = Myoglobulin (MW - 17 KD 4 = Phenylalanine

			Column: Reprosil SEC 4000, 5 µm A.) 250 x 4.6 mm, Flow: 1 ml / min B.) 750 x 4.6 mm, Flow: 0,33 ml / min Eluent: 50 mM KH2PO4+ 150 mM KCL, pH 7.0 Sample: 1= Thyroglobulin (MW - 670 KD) 2= Ovalbumin (MW - 44 KD) 3= Phenylalanine

ReproSil 200 SEC Typical features for ReproSil 200 SEC, 5 µm (Mat..No.: R25.sec.) pH-Stability: 2 - 7.5 Pore size: 200 A° Pore volume: 1.2 ml/g Capacity: about 10 - 500 µg, depending on column-dimension and protein Max. pressure: about 200 bars







Dimension: 300 x 8 mm, Eluent: (for all shown separations) 50 mM K-phosphate-buffer, pH=7 + 150 mM KCL, Flow: 1 ml/min., Max.Pressure: 200 bars









GPC
Polymeric phases for organic GPC:
Typical dimensions: 300 x 8 mm or 600 x 8 mm iD. Other dimensions on request.

for non aquous GPC		Daltons

ReproGel PS GPC, 5 µm, 50 A°		< 1 000	rg0.050.
ReproGel PS GPC, 5 µm, 100 A°		> 3000	rg0.100.
ReproGel PS GPC, 5 µm, 500 A°		> 20 000	rg0.500.
ReproGel PS GPC, 5 µm, 1000 A°		1 000 - 40 000	rg0.103.
ReproGel PS GPC, 5 µm,10 000 A°		4 000 - 500 000	rg0.104.
ReproGel PS GPC, 5 µm, 100 000 A°		10 000 - 2 000 000	rg0.105.
ReproGel PS GPC, 5 µm, 1 000 000 A°		200 000 - 10 000 000	rg0.106.
ReproGel PS GPC, 5 µm, linear		1 000 - 1 000 000	rg0. lin.

Available particle sizes: 3, 5, 10 µm





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