 |
|
|
|
|
|
| |
 |
|
ReproSil-Pur C18-AQ
5 µm (250x4 mm)
Eluent:
MeOH/H20 (99/1) / ACN (90/10)
Flow rate: 1.5 ml/min.
Detection: 214 nm |
|
| |
|
|
|
|
|
| |
|
| |
|
|
|
|
|
| |
 |
|
ReproSil-Pur C18-AQ,
5 µm (250x3 mm)
Eluent: 20 mM KH2PO4 (pH3) / ACN (95/5)
Det.: Fluorimetr., Flow rate: 0,4 ml/min |
|
| |
|
|
|
|
|
| |
|
| |
|
|
|
|
|
| |
 |
|
Column: ReproSil-Pur
C18-AQ,
5 µm (250x4.6 mm)
Eluent: Tetrahadrofuran / MeOH / Water (25:3:72)
Det.: 247 nm, Temp.:20 C°,
Flow rate: 1 ml/min.
|
|
| |
Sample:
1=Prednison
2=Prednisolon
3=Cortisol
4=Flucortisol
5=Methylprednisolon
6=Triamcicolon (Volon)
7=Dexamethason
|
|
|
|
|
| |
|
| |
|
|
|
|
|
| |
 |
|
ReproSil-Pur C18-AQ,
5 µm (150x4mm)
Eluent: 1000 ml water:
Na-Acetate-Trihydrate 7.465 gr., Na2-EDTA 3.7 mg
+ Octanesulfonic acid sodium salt monohydrate 108,3 mg + 110 ml
MeOH,
pH=4.1 with 100% Acetic acid.
Flow rate: 1 ml/min.
Detection: elektrochemically |
|
| |
|
|
|
|
| |
|
| |
|
|
|
|
|
| |
 |
|
With integrated sample injection:
Guard: ReproSil DIBS,
12 µm (13x4 mm)
Column: ReproSil-Pur C18-AQ, 5 µm (250x3 mm)
|
|
| |
|
|
|
|
|
| |
Sample (serum): with added metablolites:
1=PEMA (5,2 ng/ml)
2=Ethosuximide (153,8 ng/ml)
3=Primidone (6,4 ng/ml)
4=Lamotrigine (10,4 ng/ml),
5=Carbamazepindiol (1,6 ng/ml)
6=10-OH-Carbamazepine
7=Phenobarbital (6,8 ng/ml)
8=Desmethylmesuximide (2,6 ng/ml)
9=Carbamazepinepoxide (9,8 ng/ml)
10=Oxycarbamazepine
11=Phenytoine (3,9 ng/ml)
12=Carbamazepine (10,2 ng/ml) |
|
|
|
| |
|
|
|
|
|
| |
|
| |
|
|
|
|
|
| |
 |
|
ReproSil-Pur C18-AQ,
5 µm(125x4mm)
Eluent: ACN / MeOH / Acetic acuid / Water (40/80/51/900)
Flow rate: 1.5 ml/min, Detection: 275 nm
Temperature: 30 C°
Injection: 10 µl |
|
| |
|
|
|
|
|
| |
|
| |
|
|
|
|
|
| |
 |
|
ReproSil-Pur C18-AQ,
5 µm (250x4 mm)
Eluent: 0,25%
Phosphric acid,
Flow: 1ml/min.,
Tem.:30 C°,
Injection: 20 µl,
Det.: 214 nm
|
|
| |
|
|
|
|
|
| |
|
| |
|
|
|
|
|
| |
 |
|
Native Amino-Acids
ReproSil-Pur C18-AQ
5 µm (150x4.6 mm)
Eluent: H20
Temperature: 40 C°
Flow rate: 0,6 ml/min.
Detection: RI
|
|
| |
1=Alanine
2=Valine
3=Isoleucine
4=Leucine
|
|
|
|
|
| |
|
|
|
|
|
| |
|
| |
|
|
|
|
|
| |
 |
|
ReproSil-Pur C18-AQ,
5 µm (100x8 mm) + Guard (30x8mm)
Eluent: 0.1 M TEAA / ACN Gradient: Start: 15/85 in 13 min to 30/70
|
|
| |
|
|
|
|
|
| |
|
| |
|
|
|
|
|
| |
 |
|
ReproSil-Pur C18-AQ,
5µm, (125x4 mm)
Eluent: 100g NaH2PO4/H3PO4
Buffer, pH=2,1 + 306 g MeOH + 85 g Ethylacetate
Flow rate: 1,7 ml/min
Detection: 590 nm
|
|
| |
|
| |
|
| |
|
|
|
|
|
| |
 |
|
Column: ReproSil-Pur C18-AQ, 5 µm
(150 x4mm)
Eluent:
6,9g NaH2PO4 + 37 mg EDTA + 150 mg Na-Octansulfonate + 60 ml ACN + 5 ml THF in 1000 ml H20
Temperature: ambient, Detection: Electrochemical |
|
| |
Sample:
1=Noradrenaline,
2=3,4-Diphydroxyphenyl acetic Acid,
3=Homovanillic acid,
4=Dopamine
5=Epinin
6=Homovanillic acid
7=3-O-Methyldopa
|
|
|
|
| |
|
|
|
|
|
| |
|
| |
|
|
|
|
|
| |
 |
|
Column:
ReproSil-Pur C18-AQ,
5 µm, (250 x 4.6 mm)
Eluent: Water
Flow rate: 1 ml/min.
Detection: 210 nm |
|
| |
|
|
|
|
|
| |
|
| |
|
|
|
|
|
| |
 |
|
Column: ReproSil-Pur C18-AQ,
5 µm (250 x 4 mm)
Eluent:
1=100 mM Na-Phosphate, pH=3.0 B=ACN,
Gradient:20 min, 1% B, then 15 min. 15% B
Detection:210nm, Injection: 50 - 200 µl/ml |
|
| |
|
|
|
|
|
| |
1=Noradrenaline,
2=Adrenaline,
3=D/L-3-4-Dihydroxyphenyl-acetic-Acid
4=3,4-Diphydroxyphenylalanine,
5=Dopamine,
6=Tyrosine
7=D/L-Methoxy-4-Hydroxymandelic Acid,
8=Phenylalanine,
9=4-Hydroxy-3-Methoxyphenylglycol,
10=5-Hydroxyindole-3-acetic-Acid
11=Vanillic Acid
12=Homovanillic Acid |
|
|
|
| |
|
|
|
|
|
| |
|
| |
|
|
|
|
|
| |
1=Cortisone
2=Hydroxycortisone
3=Corticosterone
4=Cortisone Acetate |
|
ReproSil-Pur C18-AQ,
5 µm 125x4 mm
Eluent: H20 /MeOH (4/6)
Flow rate: 0,8 ml/min. |
|
| |
|
|
|
|
|
| |
|
| |
|
|
|
|
|
| |
 |
|
Carbamates
Column: ReproSil-Pur C18-AQ, 5 µm
(250 x 3 mm)
Eluent: A=H20 (0,5% H3PO4) B=ACN
Gradient: 4:1 to 1:4 in 30 Min. Flow: 0.5 ml/min. Injection: 5 µl Detection: 243 nm
|
|
| |
1=Fenuron
2=Metoxuron
3=Monolinuron
4=Chlortoluron
5=Isoproturon
6=Fluometuron
7=Monuron
8=Diuron
9=Metobromuron
10=Linuron
11=Diethofencarb
12=Fenoxycarb
|
|
|
|
| |
|
|
|
|
|
| |
|
| |
|
|
|
|
|
| |
 |
|
Pesticides (Urea-Derivates)
Column: ReproSil-Pur ODS-3, 5 µm (150 x 4,6 mm)
Eluent: A=H20 B=ACN
Gradient:0 -3,5 min. (ACN/H20, 40/60) isocratic
3.5 min. - 10.5 min: from 40% to 90% ACN
10.5 min, - 13.5 min. ACN/H20 (90/10) isocratic
Flow rate: 1 ml / min.
Injection: 20 µm
Detection: 210 nm |
|
| |
1=Fenuron
2=Metoxuron
3=Monolinuron
4=Chlortoluron
5=Isoproturon
6=Fluometuron
7=Monuron
8=Diuron
9=Metobromuron
10=Linuron
|
|
|
|
| |
|
| |
|
|
|
|
|
| |
 |
|
Organic Acids
ReproSil-Pur C18-AQ
5 µm, 200 x 4.6 mm
Eluent: 200 mmol/L
(20 g 85% H3PO4/l)
Temperature: 20 C°,
Detection: 230 nm |
|
| |
|
|
|
|
|
| |
|
| |
|
|
|
|
|
| |
back |
|
|
|
| |
|
|
|
|
 |
