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Column: Stability Polyamine,
5 µm (53 x 4.6 mm)
Eluent:
A= 20 mM TRIS-pH7
B=1M NH4OAc-pH7
Gradient: 0-100% B in 20 min.
Detection: 280 nm,
Flow rate: 1 ml/min
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Column: Stability Polyamine,
5 µm
Detection UV-DA
250 x 4.6 mm
Eluent: ACN/H20 (80/20)
Flow rate: 1 ml/min |
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Sorbitol and Sugars: Stability Polyamin, 5 µm (NH2 on Silical)
(250 x 4,6 mm)
Eluent: ACN/H20 (80/20), Flow rate: 1 ml/min., Detection: UV-DA |
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Sample:
1=Fructose
2=Sorbitol
3=Glucose
4=Saccharose
5=Maltose
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