High Performance LC
ReproSil SEC - for Size Exclusion


Reprosil SEC phases are designed for the Gel Filtration Chromatography (GFC) of proteins, peptides and
other org. polymers in aqueous systems. In GFC elution order is a function of molecular weight, the largest
molecules eluting first and the smallest last.
The Silica-based Reprosil SEC packings are available with 3 different pore sizes:

         
  Packing: Pore size: MW of Proteins: MW of Linear Molecules:  
        (denaturated proteins, peptides, other org. Polymers)  
 
  Reprosil 50 SEC 50 A° 8 000 - 30 000 D 500 - 10 000 D  
  Reprosil 200 SEC 200 A° 10 000 - 500 000 D 2 000 - 70 000 D  
  Reprosil SEC 4000 450 A° 20 000 - 5 000 000 D 4 000 - 500 000 D  
 
       
 
       
  Column:
ReproSil-Pur 50 SEC, 5µm (300 x 8 mm)
Flow: 0,5 ml/min.
Eluent:
50 mM KH2PO4+100mM KCL, pH6,5

Peptide Sample:

1= MW: 3894 D
2= MW: 1593 D
3= MW: 826D
  
 
       
  Column:
Reprosil 200 SEC, 5 µm
(300 x 8 mm)
Flow: 1 ml / min
Eluent:
50 mM KH2PO4+ 150 mM KCL, pH 7.0

Sample:

1 =Thyroglobulin
(MW - 670 KD)
2 = Ovalbumin
(MW - 44 KD)
3 = Myoglobulin
(MW - 17 KD
4 = Phenylalanine
 		 
 
       
  Column:
Reprosil SEC 4000, 5 µm
A.) 250 x 4.6 mm,
Flow: 1 ml / min

B.) 750 x 4.6 mm,
Flow: 0,33 ml / min

Eluent:
50 mM KH2PO4+ 150 mM KCL, pH 7.0

Sample:

1= Thyroglobulin
(MW - 670 KD)
2= Ovalbumin
(MW - 44 KD)
3= Phenylalanine
  
 
       
  ReproSil 200 SEC

Typical features for ReproSil 200 SEC, 5 µm (Mat..No.: R25.sec.)
pH-Stability: 2 - 7.5
Pore size: 200 A°
Pore volume: 1.2 ml/g
Capacity: about 10 - 500 µg, depending on column-dimension and protein
Max. pressure: about 200 bars		    
 
       
   
 
       
   
 
       
   
 
  Dimension: 300 x 8 mm, Eluent: (for all shown separations) 50 mM K-phosphate-buffer, pH=7 + 150 mM KCL, Flow: 1 ml/min., Max.Pressure: 200 bars  
   
        
  Polymeric phases for organic GPC:    
  Typical dimensions: 300 x 8 mm or 600 x 8 mm iD. Other dimensions on request.  
           
  for non aquous GPC Daltons    
 
  ReproGel PS GPC, 5 µm, 50 A° < 1 000 rg0.050.  
  ReproGel PS GPC, 5 µm, 100 A° > 3000 rg0.100.  
  ReproGel PS GPC, 5 µm, 500 A° > 20 000 rg0.500.  
  ReproGel PS GPC, 5 µm, 1000 A° 1 000 - 40 000 rg0.103.  
   ReproGel PS GPC, 5 µm,10 000 A° 4 000 - 500 000 rg0.104.  
  ReproGel PS GPC, 5 µm, 100 000 A° 10 000 - 2 000 000 rg0.105.  
  ReproGel PS GPC, 5 µm, 1 000 000 A° 200 000 - 10 000 000 rg0.106.
  ReproGel PS GPC, 5 µm, linear 1 000 - 1 000 000 rg0. lin.  
 
     
  Available particle sizes: 3, 5, 10 µm      
           
     
 
       
 
 
       
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