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Reprosil Chiral-PS is a so called brush-type phase like the other chiral Reprosil phases:
-Reprosil Chiral-NR: With a very broad generality for aromatic compounds with a Oxygen
near the chiral centre. For Normal- and Reversed phases separations.
-Reprosil Chiral-OH: for aromatic compounds with an OH-group on the chiral centre
-Reprosil Chiral-CA for aromatic compounds with a Carboxylic Acid group and
-Reprosil Chiral-AA for all Amino Acids and Oxazolidinones, Hydantoins, Diazepines and
Sulfoxides.
Most successfully separated substances with Reprosil Chiral-PS are Amides, Alcohols, Esters, Ketones, Acids, Sulfoxides, Phosphine oxides, Selenoxides, Phosphonates, Thiophosphineoxides, Phophineselenides, Phophineboranes, ß-Latames, Organometaliccs, Atropisomers and Heterocycles.
Due to the 100A° pores and the big surface area of 350 m2/g the phase has an excellent preparative capacity. With a 250 x 10 mm column one can separate 50 – 250 mg / injection and with a 250 x 20 mm column 250 – 1000 mg/ injection.
The phase is stable in all commonly used NP eluents. Typical eluents are mixtures of Hexane / IPA or Hexane / CH2Cl2 or CH2Cl2 / IPA. With Ammonium acetate one can improve the separation of acides and bases.
The best pH-range is between 2 und 7. The columns are stable up to 300 bar.
Examples for separations:
The typical column dimension is 250 x 4.6 mm with a flow of 1.5 ml / min.
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Phosphorus / Sulfur Compounds: |
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Benzyl-Methyl-Phenyl-Phosphinoxid: |
Eluent: CH2Cl2 / IPA (90/10) |
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t-Butyl-Methyl-Phenyl-Phosphinselenid: |
Eluent: Hexan / CH2Cl2 (70/30) |
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t-Butyl-Methyl-Phenyl-Phosphinoxid: |
Eluent: CH2Cl2 / IPA (90/10) |
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Chlorthalidon: |
Eluent: CH2Cl2 / MeOH (99/1) + 0,01 M Amm.acetat |
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Methyl-(2-Methylnaphtyl)-Phenyl-Phosphinoxid: |
Eluent: CH2Cl2 / IPA (75/25) |
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Methyl-Phenyl-Vinylbenzyl-Phosphinoxid: |
Eluent: Hexan / Dioxane / IPA (42/42/17) |
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Methyl-Phenyl-(2-Vinylbenzylol)-Phosphinoxid: |
Eluent: Hexan / Dioxane / IPA (38/37/25) |
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p-Methoxyphenyl-Methyl-Sulfoxid: |
Eluent: CH2Cl2 / Dioxan / Hexane (40/40/20) |
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p-Methoxyphenyl-Phenyl-Sulfoxid: |
Eluent: CH2Cl2 / IPA (98/2) |
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2-Naphtyl-Methyl-Sulfoxid: |
Eluent: CH2Cl2 / Dioxan / Hexane (28/27/45) |
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Sulpirid: |
Eluent: CH2Cl2 / Ethanol (95/5) + 0,01 M Ammioniumacetate |
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p-Toluyl-Methyl-Sulfoxid: |
Eluent: CH2Cl2 / IPA (98/2) |
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4-(m-Xylyl)-Benzyl-Sulfoxid: |
Eluent: CH2Cl2 / IPA (95/5) |
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Other compounds : |
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Fluazifop: |
Eluent: Hexane / CH2Cl2 / IPA (90/9/1) |
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Fluazifop-Butylester: |
Eluent: Hexane / IPA (95/5) |
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Haloxyfop-Ethoxyethylester: |
Eluent: Hexane / IPA (95/5) |
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Quizalofop-Ethyester: |
Eluent: Hexane / IPA (95/5) |
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ß-Lactam: |
Eluent: Hexane / CH2Cl2 / IPA (48/48/4) |
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Examples with Reprosil Chiral-PS, 8 µm (r18.ps.s2546) |
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Flow: 1 ml / min. |
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Det. 254 nm |
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