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Features:
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- C18 monolayer bonded to spherical particles with 100 A° pores, 450 m2/g and a pore volumne
of 1.1 ml/g
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- Impurities for metals: Al, Ti, Fe, Zr: all < 1 ppm
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- Typical plates / m between 80 and 100 000 for 5 µm
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- pH-stable from 1 up to 10 because of
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- double bound endcapping: unique stable
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Each batch is validated with following 6 quality tests:
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Test 1:
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Inactivity of Silanols:
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k (Pyridine) / k (Phenyl) |
Specification: < 0.45 |
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(eluent: MeOH / water, 7/3
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Test 2: |
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Retentivity for Hydrophilic Compounds: |
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(column 150 x 4.6 mm, 1 ml flow / min.) |
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(eluent: 100 % water) |
Specification: > 9 min. |
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Test 3:
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Hydrogen-Bonding Capacity:
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(eluent: MeOH / water, 8/2)
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k (Coffeine) / k (Phenol) |
Specification: 0.5 –0.6 |
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Hydrophobicity:
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k (n-Amylbenzene) / k (n-Butylbenzene) |
Specification:145 – 1.50 |
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Shape-Selectivity:
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k (Triphenylene) / k (o-Terphenyl) |
Specification: 1.20 – 1.30 |
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Test 4:
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Peak Symmetry for Basic Compounds:
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(eluent: 20 mM K-Phosphate buffer pH 7 / ACN)
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As (Propranol) |
Specification: <1.3 typical: 1.0 – 1.1 |
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As (Amitriptyline) |
Specification: < 1.6 typical: 1.1 – 1.3 |
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Test 5:
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Peak Symmetry for Acidic Compounds: |
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(eluent: 20 mM K-Phosphate buffer pH 7 / ACN) |
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k (p-Toluic Acid / k (Methyl Benzoate) |
Specification: < 0.45 |
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As (p-Ethylbenzoic acid) |
Specification: < 1.4 typical: 0.9 – 1.1 |
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Test 6: |
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Metal Sensivity: |
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(eluent: ACN / water, 1/1) |
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As (2,3 Dihydroxynaphthalene) / AS (2,7 Dihydroxynaphtalene) 1.5 typical: 0.9 – 1.1
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Comparison of Inertsil ODS-3V and Reprosil-Pur Basic C18 |
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Comparison of different C18 Phases
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more |
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up |
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